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Assessment of calcium ions diffusion and pH measurements of three intracanal medicaments through dentinal tubules (in vitro comparative study)

*Chrakhan A-Latif  A-Qadir & **Salam D. Al-Qaisi

*College of Medicine, University of Sulaimani

**College of Dentistry, University of Sulaimani



Objectives: The purpose of this study was to evaluate the release of Calcium ions from three intracanal medicaments [Ca(OH)2 , White Proroot MTA , Biodentine], and to measure the PH of these three materials.

Materials and Methods:
Forty-two single rooted permanent teeth were prepared using crown down technique with Protaper rotary files to a master apical file size 40. The canals were irrigated by using 2 ml 5.25% NaOCl for 60 seconds after each instrument change, final irrigation of canals using 5 ml of 17% EDTA for 3 minutes and 5 ml of 5.25% NaOCl for 60 seconds and finally 10 ml of saline solution. The canals were dried and the intracanal medicaments were applied according to manufacturer instruction after setting of materials, both apical and coronal openings were sealed by Cavit and a layer of Epoxy resin on it. Each root was immersed in 30 ml of deionized water. The digital pH meter was used for measuring the pH values for each root 10 times per each studied period (1, 2, 7, 15, 30, 45, 60) days; The Calcium ions release measurements were taken for each studied periods using Atomic Absorption Spectrophotometer device. The results were analyzed statistically using ANOVA (F test), a p-value < 0.05 was considered as a significant.

The pH values for Ca (OH)2 and PMTA groups were gradually increased and need more time for increasing than the Biodentine. The pH values for Biodentine were the highest at the first day then decreased in the 2nd and 7th days, then increased over time until sixty days. The calcium ions release values were gradually increase for three medicament and its values were highest for Biodentine followed by Ca(OH)2 and finally PMTA. The differences between groups were statistically highly significant. 

Biodentine exhibited highest mean values in the calcium ions release and pH measurements than other groups and PMTA showed lowest mean values in the calcium ion release and pH measurement.

Calcium hydroxide, Proroot MTA, Biodentine, deionized water


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