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Evaluation of the antimicrobial effects of ozonated water on the sanitization of endodontic files contaminated with C. Albicans

*Niaz H. Ghareeb H. Saeed, **Chiman H. Fattah, **Kawa A. M. Amin & **Shakhawan Kadir Kadir

*College of Dentistry, Hawler Medical University.

**College of Medicine, University of Sulaimani.



Objectives: The aim of this study was to evaluate the effectiveness of 0.5 ppm ozonated water in the elimination of Candida albicans, from endodontic files. 

Materials and Methods:
The study was performed on 20 K-files, 21 mm long and of size 25. Of these, five files were taken as a control group, and the remaining 15 files were divided into three groups of 5 files each and they were tested for the efficacy of sanitization with different disinfecting material: sodium hypochlorite alone, sodium hypochlorite followed by ozonated water and ozonated water alone. From the prepared sample of C.albicans, the 20 K-files were contaminated.

: Antimicrobial effectiveness was evaluated by the reduction of microbial counts. Incomplete disinfection of ozonated water up to 20% was observed (%80 sterilization). 

: The results showed that disinfecting and exposing to ozonated water alone cannot give complete sterilization. Sodium hypochlorite followed by ozonated water can be used as an alternative method for using ozonated water alone.

Keywords: Ozonated water, endodontic files , Candida Albicans.


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