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sdj-10061

Perilesional sites of OSCC had altered P-cadherin expression

Dena N. Mohammad

College of Dentistry, University of Sulaimani

DOI: https://doi.org/10.17656/sdj.10061


Abstract

Epithelial cells at surgical free margins are considered to be free of pathology at the morphological level. However, they might be associated with molecular changes when considering clonal expansion of pre-neoplastic cells in a particular tumor field; that subsequent genomic changes could drive them towards the malignant development.To evaluate the distribution and cellular localization of P-cad at the perilesional area of OSCC, in comparison with normal oral mucosa and correlate such expression to clinical parameters and histopathological grading of the primary lesion.Normal oral mucosa revealed membranous basal and parabasal expression. All the perilesional samples showed positive results with 55% overexpression. The cytoplasmic expression alone or mixed with membranous localization was the predominant expression (18 out of 20 cases). Sex, age, and clinical presentations did not show any significant relations in response to P-cad distribution or intracellular localization. While P-cad intracellular localization was significantly correlated with the histopathological grading (P=0.046) and epithelial cell layers distribution (P=0.026). P-cad overexpression was seen in 81.8% in WDSCC (9/11) perilesional sites.P-cadherin overexpression and shifting its intracellular localization from membranes to cytoplasmic and mixed at the perilesional site of oral cancer could be considered as a mark for revealing cancerization and lateral spreading. 

Keywords: P-cad, perilesional, overexpression, intracellular localization.


References

1.YardimciG,Kutlubay  Z, Engin B, TuzunY.Precancerous lesions of oral mucosa. World J Clin Cases. 2014 ; 2(12): 866-872.

2.    Mohan  M, Jagannathan N. Oral field cancerization: an update on current concepts.Oncol Rev .2014; 8:244.

3.    MessadiVD.          Diagnostic                   aids            for              detection    of               oral precancerous conditions. Inte J Oral Sci. 2013; 5:59–65.

4.    Ashwini  KB, Sharada  P, Hema  NK, Chitra  MS. Oral field cancerization: An update of evidences. J NTR Univ Health Sci.2015;4 (3):141-4.

5.    Wisniewski JDM, Ma T and Schneider A. Advances in the chemopreventive targeting of oral carcinogenesis.Curr Oral Health Rep. 2015 ;  2:63–72.

6.    Jaiswal G, Jaiswal S, Kumar R, Sharma A. Field cancerization: concept and clinical implications in head and neck squamous cell carcinoma.JExpTherOncol. 2013;10(3):20914.

7.    Tsao AS, Kim ES, Hong WK. Chemoprevention of cancer. CA Cancer J Clin. 2004; 54(3):150-80.

8.    Dakubo DG, Jakupciak PJ, Birch-Machin AM, and Parr LR. Clinical implications and utility of field cancerization. Cancer Cell Int. 2007; 7: 2.

9.    Braakhuis BJ, Tabor MP, Kummer JA, Leemans CR,

Brakenhoff RH. A genetic explanation of Slaughter's concept of field cancerization: evidence and clinical implications. Cancer Res .2003; 63:1727–1730.

10.  Bauer K, Gosau M, Reinders J,  Oefner P,  Reichert ET and  Bauer R. Presenilin 1/γ-secretase modulates P-cadherin processing and influences cell adhesion in oral squamous cell carcinoma cell lines. Carcinogenesis .2013;34 (11): 2622-2628.

11.  PyoSW,HashimotoM,Kim YS, Kim  CH, Lee SH, Johnson KR, Wheelock M J and Park JU. Expression of E-cadherin, Pcadherin and N-cadherin in oral squamous cell carcinoma: correlation with the clinicopathologic features and patient outcome. J CraniomaxillofacSurg.2007; 35:1-9.

12.  Hashimoto T, Soeno Y, Maeda G, Taya Y, Aoba T, Nasu M, KawashiriS,Imai K. progression of oral squamous cell  carcinoma accompanied with reduced E-cadherin expression but not cadherin switch. PLoS ONE. 2012; 7(10): e47899.

13.  Lo Muzio L, Pannone G, Mignogna MD, Staibano S, Mariggiò MA, Rubini C, Procaccini M, Dolci M, Bufo P, De Rosa G and Piattelli A. P-cadherin expression predicts clinical outcome in oral squamous cell carcinomas. HistolHistopathol. 2004; 19:1089-99.

14.  Lo Muzio L, Campisi G, Farina A, Rubini C, Pannone G, Serpico R,.LainoG, De Lillo A and Carinci F. P-cadherin expression and survival rate in oral squamous cell carcinoma: an immunohistochemical study. BMC Cancer. 2005; 5:63.

15.Bauer R ,Dowejko D, Driemel O, Boßerhoff AK and Reichert TE .Truncated P-cadherin is produced in oral squamous cell carcinoma .FEBS J .2008; 275: 4198–4210.

16.Chai H and Brown ER. Field effect in cancer–An update. Ann Clin Lab Sci. 2009 ; 39 (4) :331-337.

17.Kato K, Hara A, Kuno T, et al: Aberrant promoter hypermethylation of p16 and MGMT genes in oral squamous cell carcinoma and the surrounding normal mucosa. J Cancer Res ClinOncol. 2006;132: 735-743.

18.Ruesga MT, Acha-Sagredo A, Rodríguez MJ, et al: p16INK4a promoter hypermethylation in oral scrapings of oral squamous cell carcinoma riskpatients. Cancer Lett. 2007; 250: 140-145.

19.             Yeh KT, Shih MC, Lin TH, et al: The correlation between CpGmethylation on promoter and protein expression of Ecadherin in oral squamous cellcarcinoma. Anticancer Res .2002; 22: 3971-3976.

20.             Díez-Pérez R, Campo-Trapero J , Cano-Sánchez J., LópezDuránM,.Gonzalez-Moles M.A,. Bascones-Ilundain J and Bascones-Martinez A.Methylation in oral cancer and precancerous lesions (Review). Oncol Rep. 2011; 25: 1203-1209.

21.             Aparna M, Shenai P, Chatra L, Veena KM, Rao KP, Prabhu VR, ShahinKA.Fieldcancerization: A review. Arch Med Health Sci.2013 ; 1 (2):136-9.

22.             Mohammad  ND,. Garib TB, Khudeir HH & Abdul–Majeed AB. Immunohistochemical expression of P-cadherin and cortactin in oral squamous cell carcinomas. Sulaimani Dent J. 2015;2(1):8-14.

23.             Pérez-Sayáns M, Somoza-Martín JM, Barros-Angueira F, Reboiras-López MD, Gándara Rey JM, García-García A.  Genetic and molecular alterations associated with oral squamous cell cancer (Review).Oncol Rep. 2009 ;22(6):1277-82.

24.             Feller LL, Khammissa  RR, Kramer BB and Lemmer  JJ .Oral squamous cell carcinoma in relation to field precancerisation: pathobiology.Cancer Cell Inte. 2013; 13:31.

25.             Williams H.K, Sanders D.S A, Jankowski JA Z, Landini G. and Brown A.M S. Expression of cadherins and catenins in oral epithelial dysplasia and squamous cell carcinoma. J. Oral Pathol. 1998; 27:308-317.