sdj-10066

Issues‎ > ‎SDJ, Vol.4, No.1, 2017‎ > ‎

Relationships of Candida with Frequency of Brushing, Age and Smoking in Patients with Removable Dental Prosthesis: A Quantitative Study

*Alan N. Ghalib, **Sarhang S. Gul , **Jwan F. Abdulkareem

*Department of Microbiology, College of Medicine, University of Sulaimani, Sulaimani, Iraq.

**

Department of Periodontics, College of Dentistry, University of Sulaimani, Sulaimani, Iraq.

**

Department of Prosthodontics, College of Dentistry, University of Sulaimani, Sulaimani, Iraq.

DOI: https://doi.org/10.17656/sdj.10066

Abstract

Objective: Although progress has been made to reduce opportunistic infection of fungi in the oral cavity, the prevalence of denture stomatitis associated with Candida has increased. The purpose of this study was to evaluate the effect of denture cleaning frequencies, age and smoking habit on the levels of Candida species in the saliva of patients wearing removable partial or complete dentures.

Methods: Subjects wearing removable partial or complete dentures were recruited. A questioner was used to collect information on oral hygiene habits and smoking. Saliva samples were collected by oral rinse technique in a sterile container and cultured in duplicate Sabouraud Dextrose Agar. The numbers of colonies were determined by aCOLyte colony counter and the number expressed as a colony forming unit (CFU). The CFU and clinical data were analyzed for correlation and the Kruskal-Wallis test was used to determine statistically significant differences.

Results: Among 99 subjects recruited, 47 were wearing complete dentures and 28 were smokers. The brushing frequencies were once (37 subjects), twice (39 subjects) and three times (23 subjects) per day. CFU was significantly higher in partial denture wearers than complete denture wearers and the brushing frequencies significantly correlated with CFU (r = -0.85, P= 0.001). There was no statistically significant difference in CFU between smokers and non-smokers and no correlation of CFU with age was found.

Conclusions: This quantitative study has suggested that there are statistically significant differences in the levels of Candida in the saliva of subjects with different brushing frequencies and wearing a different type of denture. However, no statistically significant difference was noticed between smokers and non-smokers, and there was no significant correlation between CFU and age.

Keywords: Levels of Candida, Saliva, Denture wearer, Cleaning frequencies.

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References:

1. Hauman CH, Thompson IO, Theunissen F, Wolfaardt P. Oral carriage of Candida in healthy and HIV-seropositive persons. Oral Surg Oral Med Oral Pathol. 1993;76(5):570-2.

2. Salvatori O, Puri S, Tati S, Edgerton M. InnateImmunity and Saliva in Candida albicans-mediated Oral Diseases. J Dent Res. 2016;95(4):365-71.

3. Martori E, Ayuso-Montero R, Martinez-Gomis J,Vinas M, Peraire M. Risk factors for denture-related oral mucosal lesions in a geriatric population. J Prosthet Dent. 2014;111(4):273-9.

4. Williams DW, Kuriyama T, Silva S, Malic S, Lewis MA. Candida biofilms and oral candidosis: treatment and prevention. Periodontol 2000. 2011;55(1):250-65.

5. Pires FR, Santos EB, Bonan PR, De Almeida OP, Lopes MA. Denture stomatitis and salivary Candida in Brazilian edentulous patients. J Oral Rehabil. 2002;29(11):1115-9.

6. Chandra J, Mukherjee PK, Leidich SD, Faddoul FF, Hoyer LL, Douglas LJ, et al. Antifungal resistance of candidal biofilms formed on denture acrylic in vitro. J Dent Res. 2001;80(3):903-8.

7. Gendreau L, Loewy ZG. Epidemiology and etiology of denture stomatitis. J Prosthodont 2011;20(4):251- 60.

8. Soysa NS, Ellepola AN. The impact of cigarette/tobacco smoking on oral candidosis: an overview. Oral Dis. 2005;11(5):268-73.

9. Ramage G, Tomsett K, Wickes BL, Lopez-Ribot JL, Redding SW. Denture stomatitis: a role for Candida biofilms. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2004;98(1):53-9.

10. Webb BC, Thomas CJ, Willcox MD, Harty DW, Knox KW. Candida-associated denture stomatitis. Aetiology and management: a review. Part 2. Oral diseases caused by Candida species. Aust Dent J. 1998;43(3):160-6.

11. Samaranayake LP, Lamb AB, Lamey PJ,MacFarlane TW. Oral carriage of Candida species and coliforms in patients with burning mouth syndrome. J Oral Pathol Med. 1989;18(4):233-5.

12. Sakashita S, Takayama K, Nishioka K, Katoh T. Taste disorders in healthy "carriers" and "non-carriers" of Candida albicans and in patients with candidosis of the tongue. J Dermatol. 2004;31(11):890-7.

13. Nair PN, Sjogren U, Krey G, Kahnberg KE, Sundqvist G. Intraradicular bacteria and fungi in root-filled, asymptomatic human teeth with therapy-resistant periapical lesions: a long-term light and electron microscopic follow-up study. J Endod.1990;16(12):580-8.

14. Samaranayake LP, MacFarlane TW. Oral candidosis. London: Wright; 1990.

15. Global Burden of Disease Study C. Global, regional, and national incidence, prevalence, and years lived with disability for 301 acute and chronic diseases and injuries in 188 countries, 1990-2013: a systematic analysis for the Global Burden of Disease Study

2013. Lancet. 2015;386(9995):743-800.

16. Harriott MM, Noverr MC. Importance of Candida- bacterial polymicrobial biofilms in disease. Trends Microbiol. 2011; 19(11):557-63.

17. Sanchez-Vargas LO, Estrada-Barraza D, Pozos- Guillen AJ, Rivas-Caceres R. Biofilm formation by oral clinical isolates of Candida species. Arch Oral Biol. 2013;58(10):1318-26.

18. Pereira-Cenci T, Cury AA, Cenci MS, Rodrigues- Garcia RC. In vitro Candida colonization on acrylicresins and denture liners: influence of surface free energy, roughness, saliva, and adhering bacteria. Int J Prosthodont. 2007;20(3):308-10.

19. Radford DR, Sweet SP, Challacombe SJ, Walter JD. Adherence of Candida albicans to denture-base materials with different surface finishes. J Dent. 1998;26(7):577-83.

20. Pereira-Cenci T, Del Bel Cury AA, Crielaard W, Ten Cate JM. Development of Candida-associated denture stomatitis: new insights. J Appl Oral Sci: revista FOB. 2008;16(2):86-94.

21. Figueiral MH, Azul A, Pinto E, Fonseca PA, Branco FM, Scully C. Denture-related stomatitis: identification of aetiological and predisposing factors - a large cohort. J Oral Rehabil. 2007;34(6):448-55.

22. Gusmão JM, Ferreira dos Santos SS, Neisser MP, Jorge AO, Faria MI. Correlation between factors associated with the removable partial dentures use and Candida spp. in saliva. Gerodontology. 2011;28(4):283-288.

23. Abu-Elteen KH, Abu-Alteen RM. The prevalence of Candida albicans populations in the mouths of complete denture wearers. New Microbiol. 1998;21(1):41-8.

24. Shin ES, Chung SC, Kim YK, Lee SW, Kho HS. The relationship between oral Candida carriage and the secretor status of blood group antigens in saliva. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2003;96(1):48-53.

25. Masipa JN, Hauman CH, Raubenheimer EJ. Oral carriage of Candida species in patients visiting the Medunsa Dental Clinic. J Dent Assoc S Afr. 1992;47(9):407-9.

26. Darwazeh AM, Al-Dwairi ZN, Al-Zwairi AA. The relationship between tobacco smoking and oral colonization with Candida species. J Contemp Dent Pract. 2010;11(3):17-24.

27. Loster JE, Wieczorek A, Loster BW. Correlation between age and gender in Candida species infections of complete denture wearers: a retrospective analysis. Clin Interv Aging. 2016;21(11):1707-1714.