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sdj-10113

Evaluation of Salivary Biomarkers and Their Correlation to Periodontal Status and BMI

Tarza J. Mahmood* , Faraedon M. Zardawi*

*Department of Periodontics, College of Dentistry, Sulaimani University, Sulaimani, Iraq.

Submitted: 01/09/2020; Accepted: 10/09/2020; Published 01/12/2020

DOI: https://doi.org/10.17656/sdj.10113

Abstract

Objective: This study aimed to determine the correlation between periodontal status and BMI by evaluating salivary biomarkers - Resistin, visfatin, and TNF-α.          

Methods: Among 580 screened subjects, 86 subjects (30 male and 56 female) aged 30-60 years recruited and grouped into four groups, Group 1 – non-obese with healthy periodontium, group 2 – non-obese with periodontitis, group 3 - obese with healthy periodontium, and group 4 - obese with periodontitis, according to their clinical periodontal parameters and BMI. (ELISA) was used to measure the salivary concentration of Resistin, Visfatin, and TNF-α. (SPSS, v.20.0) applied for statistical analysis, Shapiro-Wilk used to test the Normality of distribution. One-way ANOVA and Mann-Whitney U test were used to analyze the outcome data.

Results: Significant differences in the clinical variables (PI, BI, PPD, and CAL) between test and control groups, with and with no periodontitis (P<0.05). However, no significant differences (P>0.05) between the level of Resistin were detected in the four groups tested. TNF-α was and periodontitis obese groups (P-value< 0.05). No significant correlations were found between clinical periodontal parameters and BMI and the three biomarkers' levels in the four groups tested in this study.

Conclusions: The study failed to detect significant positive correlations between the salivary biomarkers' levels and clinical periodontal and obesity parameters.

                                                                                                                                                                                                                                                                                                                                             

Keywords: BMI, Obesity, Periodontitis, Salivary biomarker.                                                                                                                                                                                                                                                Full Article - PDF                                                                                                                                                                                                                                   
                                                                                                                                                                                                                                                                                                                                                   

References:

1. Silva N, Abuleme L, Bravo D, Dutzan N, Garcia- sensich J, Vernal R, et al. Host response mechanisms in periodontal diseases. J Appl Oral Sci. 2015;23(3):329-55.
2. Krejci CB, Bissada NF. Obesity and periodontitis: a link. Gen Dent. 2013;61(1):60-3.
3. Cardoso EM, Reis C, Manzanares-sespedes MC. Chronic periodontitis, inflammatory cytokines, and interrelationship with other chronic diseases. J Postgrad med. 2018;130(1):98-104.
4. Teles FR, Teles RP, Martin L, Socransky SS, Haffajee AD. Relationships among interleukin-6, tumor necrosis factor-α, adipokines, vitamin D, and chronic periodontitis. J Periodontol. 2012;83(9):1183-91.
5. Gokhale NH, Acharya AB, Patil VS, Trivedi DJ, Setty S, Thakur SL. Resistin levels in gingival crevicular fluid of patients with chronic periodontitis and type 2 diabetes mellitus. J Periodontol. 2014;85(4):610-7.
6. Saito T, Shimazaki Y. Metabolic disorders related to obesity and periodontal disease. Periodontol 2000. 2007;43:254-66. 2.
7. De Courten BV, Degawa-Yamauchi M, Considine RV, Tataranni PA. High serum resistin is associated with an increase in adiposity but not a worsening of insulin resistance in Pima Indians. Diabetes. 2004;53(5):1279-84.
8. Saito T, Shimzaki Y, Koga T, Tsuzuki M, Ohshima A. Relationship between upper body obesity and periodontitis. J Dent Res. 2001;80(7):1631-6.
9. AL-Zaharani MS, Bissada NF, Borawskit EA. Obesity and periodontal disease in young, middle- aged, and older adults. J Periodontol. 2003;74(5):610-5.
10. Genjo RJ, Grossi SG, Ho A, Nishimura F, Murayama Y. A proposed model linking Periodontal disease and obesity diabetes, and periodontal infections. J Periodontol. 2005;76:2075-84.
11. Zimmerman GS, Bastos MF, Gonҫlaves TED, Chambrone L, Duarte PM. Local and circulating levels of adipocytokines in obese and normal weight individuals with chronic periodontitis. J Periodontol. 2013;84(5):624-33.
12. Amar S, Zhou Q, Shaik-Dasthagirisaheb Y, Leeman S. Diet-induced obesity in mice causes changes in immune responses and bone loss manifested by bacterial challenge. Proc Natl Acad Sci. 2007;104(51):20466-71.
13. Lundin M, Yucel-Lindberg T, Dahllӧf G, Marcus C, Modѐer T. Correlation between TNFalpha in gingival crevicular fluid and body mass index in obese subjects. Acta Odontol Scand. 2004;62(5):273-7.
14. Saito T, Yamaguchi N, Shimazaki Y, Hayashida H, Yonemoto K, Doi Y, et al. Serum levels of Resistin and adiponectin in women with periodontitis: the Hisayama study. J Dent Res. 2008;87(4):319-22.
15. Khader YS, Bawadi HA, Haroun TF, Alomari M, Tayyem RF. The association between periodontal disease and obesity among adults in Jordan. J Clin Priodontol 2009;36(1):18-24.
16. Fukuhara A, Matsuda M, Nishizawa M, Segawa K, Tanaka M, Kishimato K, et al. Visfatin: a protein secreted by visceral fat that mimics the effects of insulin. Science 2005;307(5708):426-30.
17. Nogueira AVB, Nokhbehsaim M, Eick S, Bouarauel Ch, Jager S, Jepsen S, et al. Regulation of visfatin by microbial and biomechanical signals in PDL cells. Clin oral Investig. 2014;18(1):171-8.
18. Tolonen H, Koponen P, Arpon A, Graff-Iversen SCS, Grovedt L, Kanieff M, et al. Recommendations for the health examination surveys in Europe. 2008.
19. Papapanou PN, Sanz M, Buduneli N, Dietrich Th, Feres M, Fine DH, et al. Periodontitis: consensus report of workgroup 2 of the 2017 world workshop on the classification of periodontal and peri- implant diseases and conditions. J Periodontol. 2018;89 suppl:173-82.
20. Navazesh M. Methods for coolloecting saliva. Ann NY Acad Sci. 1993;694:72-7.
21. Ainamo J, Bay I. Problems and proposals for recording gingivitis and plaque. Int Dent J. 1975;25(4):229-35.
22. Lindhe J, Sokransky SS, Nyman S, Haffajee A, Westfelt E.“Critical probing depths” in periodontal therapy. J Clin Periodontol. 1982;9(4):323-36.
23. Desai GS, Mathews ST. Saliva as a non-invasive diagnostic tool for inflammation and insulin- resistance. World J. Diabetes. 2014;5(6):730-8.
24. Ceron JJ, Lamy E, Martinez-Subiela S, Lopez- Jornet P, Silva FCE, Eckersall PD, et al. Use of saliva for diagnosis and monitoring the SARS- CoV-2: a general perspective. J Clin Med. 2020;9(5):1491.
25. Azuma K, Katsukawa F, Oguchi Sh, Murata M, Yamazaki H, Shimada A, et al. Correlation between serum resistin level and adiposity in obese individuals. Obes Res. 2003;11(8):997-01.
26. Buduneli, N, Biyikoglu B, Ilgenli T, Buduneli E, Nalbantsoy A, Saraҫ F, et al. Is obesity a possible modifier of periodontal disease as a chronic inflammatory process? A case–control study. J Periodontol Res. 2014;49(4):465-71.
27. Dursun, E, Akalin FA, Genc T, Cinar N, Erel O, Yildiz BO. Oxidative stress and periodontal disease in obesity. Medicine (Baltimore). 2016;95(12):e3136.
28. Lehrke M, Reilly MP, Millington SC, Iqbal N, Rader DJ, Lazar MA. An inflammatory cascade leading to hyperresistinemia in humans. PLoS Med. 2004;1(2):e45.
29. Anderson PD, Mehta NN, Wolfe ML, Hinkle ChC, Pruscino L, Comiskey LL, et al. Innate immunity modulates adipokines in humans. J Clin Endocrino Metab. 2007;92(6):2272-9.
30. Esfahrood ZR, Tehrani SV, Yadegari Z, Shams B, Dehnavi F, Shams N. Evaluation of resistin levels in saliva of patients with chronic periodontitis and healthy subjects. Chin J Dent Rest. 2018;21(2):143-6.
31. Suresh S, Mahendra J, Singh G, Pradeep AR, Sundaravikram, Sekar H. Comparative analysis of GCF Resistin levels in obese subjects with and without periodontal disease. J. Clin Diag Res. 2016;10(5):p ZC71-4.
32. Chapple ILC, Mealey BL, Van Dyke ThE, Bartold PM, Dommisch H, Eickholz P, et al. Periodontal health and gingival diseases and conditions on an intact and a reduced periodontium: Consensus report of workgroup 1 of the 2017 world workshop on the classification of periodontal and peri- implant diseases and conditions. J. Periodontol. 2018;89:S74-S84.
33. Özcan E, Saygun NI, Serdar MA, Kurt N. Evaluation of the salivary levels of visfatin, chemerin, and progranulin in periodontal inflammation. J Clin Oral Investig. 2015;19(4):921-8.
34. Afacan B, Yücel ZP, Yenisey Ç, Nizam N, Emingil G. Salivary Resistin and TNF-α Levels in Different Periodontal Diseases. J Ege University School of Dentistry. 2019;40(3):177-84.
35. Pradeep AR, Raghuvendra NM, Prasad MVR, Kathariya R, Patel SP, Sharma A. Gingival Vol 7(2) Mahmood and Zardawi 35 crevicular fluid and serum visfatin concentration: their relationship in periodontal health and disease. J Periodontol. 2011;82(9):1314-9.
36. Tabari ZA, Azadmehr A, Nohekhan A, Naddafpour N, Ghaedi FB. Salivary visfatin concentrations in patients with chronic periodontitis. J Periodontol 2014;85(8):1081-5.
37. Lehmann-Kalata A, Miechowicz I, Korybalska K, Swora-Cwynar E, Czepulis N, Luczak J, et al. Salivary fingerprint of simple obesity. Cytokine. 2018;110:174-80.
38. Ziccardi P, Nappo F, Giugliano G, Esposito K, Marfella R, Cioffi M, et al. Reduction of inflammatory cytokine concentrations and improvement of endothelial functions in obese women after weight loss over one year. Circulation. 2002;105(7):804-9.
39. Syrjäläinen S, Gursoy UK, Gursoy M, Pussinen P, Pietiäinen M, Jula A, et al. Salivary Cytokine Biomarker Concentrations in Relation to Obesity and Periodontitis. J Clin Med. 2019;8(12):2152.












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