Quantitative Expression of Cell-Free Salivary Transcriptomes (IL8, IL1β, And H3F3A) in Oral Squamous Cell Carcinoma and Oral Erosive Lichen Planus

Volume 4 , Issue 1 , December 2017 , Pages 21-27

Authors

Darya K. Mahmood 1 ; Balkees T. Garib 2 ; Belal A. Muhammad 1

1 Department of Dental Nursing, Sulaimani Polytechnic University, Sulaimani, Iraq.

2 Department of Oral Diagnosis, College of Dentistry, University of Sulaimani, Sulaimani, Iraq.

DOI logo 10.17656/sdj.10067

Keywords

Abstract

Objective: Diagnosis of oral SCC at its earliest stage is crucial for increasing survival rate. Salivary transcriptome meets
demands for noninvasive, accessible, and highly efficient diagnostic medium. This study designed to investigate whether
quantification of cell-free salivary mRNA of certain genes (IL8, IL1β, H3F3A) is an informative diagnostic tool for early
detection of oral SCC and malignant transformation in erosive oral lichen planus.
Methods: The mRNA of salivary transcriptomes of IL8, IL1B, and H3F3A genes were examined in 18 individuals (six oral
SCC, six oral lichen planus, and six healthy people). Up to 5 ml of unstimulated saliva was collected from each person. The
total RNA was extracted from the cell-free saliva samples and converted to cDNA. The mRNA expression level of the indicated
genes was quantified by a qRT-PCR technique using specific primers and SyberGreen reagent. The data obtained from the
qRT-PCR reaction was analyzed using the comparative CT (2-ΔΔCT) method. Independent t-test was performed to find the
relation between variables. P-value < 0.05 was considered significant.
Results: The results show that the mRNA expression of all the three indicated genes (IL8, IL1B, and H3F3A) was up-regulated
in all OSCC and erosive oral lichen planus cases in comparison to normal cases.
Conclusions: This work demonstrates a significant correlation between cell-free salivary mRNA of certain genes and cases
of OSCC and oral lichen planus. These findings suggest that detection of salivary mRNA of certain related genes hold promises
as future biomarkers for accurate and early diagnosis of oral malignancies. 

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  • Published at1 December 2017

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