Efficacy of Controlled-Release Combination of Novel Amoxicillin and Metronidazole Nanoparticles in Experimental Periodontitis: An in Vivo Study
Volume 11 , Issue 2 , August 2024 , Pages 53-65
Authors
Nabaz Y. Hussein 1 ; Sarhang S. Gul 2 ; Sadat A. Aziz 3
1 University of Sulaimani
2 University of Sulaimani
3 University of Sulaimani
10.17656/sdj.10192
Keywords
Abstract
Objective: This study was formulated to examine the efficacy of a novel gel incorporating nanoparticles of amoxicillin and metronidazole as an adjunctive to periodontal treatment on periodontal clinical parameters.
Methods: Fifty-eight healthy male rats were used in the study. The study design includes five groups: Group 1 Baseline (Day 0): normal rats without periodontitis induction or treatment. Group 2: untreated induced periodontitis (IP); Group 3 (negative control): Scaling and root surface debridement (RSD) with chitosan gel: Group 4: Positive control: using the (1.5%) gel of the microparticle combination of amoxicillin and metronidazole; Group 5: Test group: applying the nanoparticle gel (1.5%). Experimental periodontitis was induced by ligating a silk suture around the cervical area of the lower central incisor. Periodontal clinical parameters, including gingival index (GI), alveolar bone level (ABL) (mm), bleeding index (BI), mobility index (MI), and periodontal pocket depth (PPD) (mm), were measured after 2 and 4 weeks.
Results: The novel nanoparticle combination of amoxicillin and metronidazole gel significantly improved periodontal clinical parameters of GI (2.33 ± 0.51 to 1 ± 0.63), BI (%) (76.20% ± 1.52 to 43% ± 2.21), MI (2.16 ± 0.40 to 0.66 ± 0.51), and PPD (mm) (0.81 ± 0.049mm to 0.49 ± 0.056mm) at two weeks following IP induction. However, the microparticle combination of amoxicillin and metronidazole only significantly improved MI and PPD (P<0.05). BI, MI, and PPD improved significantly in groups 4 and 5 compared to group 2 at 4 weeks.
Conclusions: These study results suggest that the novel nanoparticle gel significantly improved periodontal clinical parameters when used in this therapeutic modality.