Impact of Immunohistochemical Expression of Collagen III and MMP-14 in Odontogenic Keratocyst

Volume 10 , Issue 2 , August 2023 , Pages 27-35

Authors

Helin F. Hassan 1 ; Balkis T. Garib 1 ; Dena N. Mohammad 1

1 University of Sulaimani

DOI logo 10.17656/sdj.10171

Keywords

Abstract

Objective: Immunohistochemical expression of collagen III and MMP14 was evaluated in odontogenic keratocyst, and correlated to the clinicopathological parameters to remark on the role of these markers in the biological behavior of the cyst.
Methods: Twenty-three odontogenic keratocysts samples were collected from three pathological laboratories in Sulaimani. Demographic information and the available radiographic investigation were recorded. The immune expression of collagen III and MMP14 was evaluated and related to the clinicopathological variables. Chi-square test was used for analysis and a p-value of 0.05 was cut off point for significance.
Results: Males were predominantly affected by odontogenic keratocysts lesions (69.6%), which were mainly detected in the >35 years age groups. Keratocyst lesions were seen more frequently in the mandible (82.6%), with well-defined borders, and unilocular appearance. Collagen III was oriented more commonly in a parallel direction (70.8%), with moderate intensity (45.8%). MMP14 revealed cytoplasmic expression in the epithelial lining of the keratocyst (100%), score 3 was the most prevalent expression (54.2%) in the cystic wall, and prominent inflammation and epithelial separation were detected with high scoring of MMP14, which were (57.1%) and (56.3%) respectively.
Conclusions: Both markers had independent combined roles in the cyst’s biological behavior, including the prevalent parallel direction of the collagen III, while overexpression of the MMP14 might have an impact role in the aggressive behavior of the lesion.

References

  1. Menon S. Keratocystic odontogenic tumours: etiology, pathogenesis and treatment revisited. J Maxillofac Oral Surg. 2015;14 (3):541-7.
  2. Qu J, Yu F, Hong Y, Guo Y, Sun L, Li X, et al. Underestimated PTCH1 mutation rate in sporadic keratocystic odontogenic tumors. Oral Oncol. 2015;51 (1):40-5.
  3. The International Agency for Research on Cancer. Pathology and Genetics of Head and Neck Tumours [OP] (Medicine):9789283224174:Medicine & Health Science [email protected]. World Health Organization. 2005. p. 430.
  4. El-Naggar AK, Chan JKC, Grandis JR, Takata TSP. WHO Classification of Head and Neck Tumours. WHO classification of Head and neck Tumors 2017. 2017.p 215-220.
  5. Krishna R, Johny J, Punathil S, and Khan NM. A rare case of odontogenic keratocyst crossing lower midline. Austin J Dent. 2019;6 (1):11-25.
  6. Oral and Maxillofacial Pathology Brad W. Neville, DDS, Douglas D. Damm, DDS, Carl M. Allen, DDS, MSD, Angela C. Chi, DMD - Google Books.
  7. Bhagawati BT, Gupta M, Narang G, Bhagawati S. Keratocystic odontogenic tumor with an ectopic tooth in maxilla. Case Rep Dent. 2013;2013:1-4.
  8. Kumari K. Ghosh S. Patil S. Augustine D. Samudrala Venkatesiah S, Rao RS. Expression of type III collagen correlates with poor prognosis in oral squamous cell carcinoma. J Investig Clin Dent. 2017;8 (4):1-7.
  9. D'hondt S, Guillemyn B, Syx D, Symoens S, De Rycke R. Vanhoutte L, et al. Type III collagen affects dermal and vascular collagen fibrillogenesis and tissue integrity in a mutant Col3al transgenic mouse model. Matrix Biol. 2018;70:72-83.
  10. Nielsen MJ, Karsdal MA. Type III Collagen. Biochemistry of Collagens, Laminins and Elastin: Structure, Function and Biomarkers. Elsevier Inc. 2016. p 21-30.
  11. Kumari K. Ghosh S. Patil S. Augustine D. Samudrala Venkatesiah S, Rao RS. Expression of type III collagen correlates with poor prognosis in oral squamous cell carcinoma. J Investig Clin Dent. 2017;8 (4):1-7.
  12. Claesson-Welsh L. How the matrix metalloproteinase MMP14 contributes to the progression of colorectal cancer. J Clin Invest. 2020;130 (3):1093-5.
  13. Linklater E, Jewett CE, Prekeris R. Polarized membrane trafficking in development and disease: from epithelia polarization to cancer cell invasion. Cell Polarity Dev Dis. 2018;121-46.
  14. Gobin E, Bagwell K, Wagner J, Mysona D. Sandirasegarane S. Smith N, et al. A pan-cancer perspective of matrix metalloproteases (MMP) gene expression profile and their diagnostic/prognostic potential. BMC Cancer. 2019;19 (1):1-10.
  15. Karande AM, Khandeparkar R, Vergeese CS JH. Role of molecular markers in understanding the tumor invasioness of ameloblastoma. Med Res Chronicles. 2017;4 (1):1-9.
  16. Singh H. Shetty DC, Wadhwan V. Aggarwal P. A quantitative and qualitative comparative analysis of collagen fibers to determine the role of connective tissue stroma on biological behavior of odontogenic. Natl J Maxillofac Surg. 2012;13 (1):15.
  17. Paulo P, Santos A, Francisco C, Nonaka W. Carlos Galvão Barboza A, et al. Immunohistochemical analysis of MMP-13 and EMMPRIN in epithelial odontogenic lesions. Eur Arch Oto-Rhino- Laryngology. 2019;1 (3):3203-11.
  18. Waheed SA, Zaidan TF, Abdullah BH. Odontogenic cysts and tumors of maxilla and maxillary sinus (A clinicopathological analysis). J Baghdad Coll Dent. 2021;33 (4):38-43.
  19. Akhtar F. Gillani SM. Clinicopathological Analysis of Odontogenic Cysts in Iraqi Population. J Med Chem Sci. 2022;6 (4):61-5.
  20. Hosgor H. Tokuc B. Kan B. Coskunses FM. Evaluation of biopsies of oral and maxillofacial lesions: A retrospective study. J Korean Assoc Oral Maxillofac Surg. 2019;45 (6):316-23.
  21. Mohanty S. Dabas J, Verma A, Gupta S, Urs AB, Hemavathy S. Surgical management of the odontogenic keratocyst: A 20-year experience. Int J Oral Maxillofac Surg. 2021;50 (9):1168-76.
  22. Al-Rawi NH, Al-Siraj AK, Majeed AH. Comparison of osteoclastogenesis and local invasiveness of ameloblastoma and keratocystic odontogenic tumor. Eur J Dent. 2018:12 (1):36-42.
  23. Jung HD, Lim JH, Kim HJ, Nam W CI, Jung HD, Lim JH, et al. Appropriate follow-up period for odontogenic keratocyst: a retrospective study. Maxillofac Plast Reconstr Surg. 2021;43 (1):1-6.
  24. Karaca Ç, Dere KA, Er N, Aktaş A, Tosun E, Köseoğlu OT, et al. Recurrence rate of odontogenic keratocyst treated by enucleation and peripheral ostectomy retrospective case series with up to 12 years of follow-up. Med Oral Patol Oral y Cir Bucal. 2018:23 (4):e443-8.
  25. Malaguez GG, Munhoz EA, Rivero ERC, Rados P V. Oliveira MG. Podoplanin expression in odontogenic keratocysts associated or not associated with nevoid Basal cell carcinoma syndrome. Appl Immunohistochem Mol Morphol. 2020;28 (7):513-7.
  26. Shoaee S, Khazaei P, Mashhadiabbas F, Varshosaz M. Sharifi F, Hessari H. Association between tooth impaction and odontogenic lesions: A matched case-control study. Med J Islam Repub Iran. 2018;32 (1):334-7.
  27. Kitisubkanchana J. Reduwan NH, Poomsawat S. Pornprasertsuk-Damrongsri S. Wongchuensoontorn C. Odontogenic keratocyst and ameloblastoma: radiographic evaluation. Oral Radiol 2020 371. 2020:37 (1):55-65.
  28. Sánchez-Burgos R. González-Martín-Moro J. Pérez-Fernández E, Burgueño-García M. Clinical, radiological and therapeutic features of keratocystic odontogenic tumours: A study over a decade. J Clin Exp Dent. 2014;6 (3):259-64.
  29. Jahagirdar DS. Role of Thickness of Collagen Fibers in Odontogenic Keratocyst and Dentigerous Cyst A Picrosirius Red Polarized Microscopic Study. J Med Sci Clin Res. 2017:05 (09):28322-7.
  30. Manthapuri S. Sanjeevareddygari S. Mantha H. Oruganti R V. Reddy S. Vamshi VR. Evaluation of biological behavior of odontogenic keratocyst and orthokeratinized odontogenic cyst using picrosirius red stain: A clinicopathological retrospective study. J Dr NTR Univ Heal Sci. 2019;8 (3):206-10.
  31. Raj Y, Sekhar MSM, Shylaja S, Bhavani SN, Ramanand OV, Patha S. et al. Evaluation of the nature of collagen fibers in KCOT, dentigerous cyst and ameloblastoma using picrosirius red stain-A comparative study. J Clin Diagnostic Res. 2015;9 (11):ZCO 1-4.
  32. Aggarwal P. Saxena S. Stromal differences in odontogenic cysts of a common histopathogenesis but with different biological behavior: A study with picrosirius red and polarizing microscopy. Indian J Cancer. 2011;48 (2):211-5.
  33. Moure SP, Carrard VC, Lauxen IS, Manso PPA, Oliveira MG, Martins MD, et al. Collagen and Elastic Fibers in Odontogenic Entities: Analysis Using Light and Confocal Laser Microscopic Methods. Open Dent J. 2011;5 (1):116.
  34. Amm HM, Casimir MD, Clark DB, Sohn P MM. Matrix metalloproteinase expression in keratocystic odontogenic tumors and primary cells. Connect Tissue Res. 2014:55 (1):97-101.
  35. Ribeiro Ribeiro AL, da Costa NMM, de Siqueira AS, Brasil da Silva W, da Silva Kataoka MS. Jaeger RG, et al. Keratocystic odontogenic tumor overexpresses invadopodia-related proteins, suggesting invadopodia formation. Oral Surg Oral Med Oral Pathol Oral Radiol. 2016;122 (4):500-8.
  36. Chen KHE, Chen C. Lopez T, Radecki KC, Bustamante K, Lorenson MY, et al. Use of a novel camelid-inspired human antibody demonstrates the importance of MMP-14 to cancer stem cell function in the metastatic process. Oncotarget. 2018:9 (50):29431-44.
  37. Lund DK, Mouly V, Cornelison DDW. MMP-14 is necessary but not sufficient for invasion of three- dimensional collagen by human muscle satellite cells. Am J Physiol - Cell Physiol. 2014;307 (2):140-9.
  38. Fuchigami T, Ono Y, Kishida S, Nakamura N. Molecular biological findings of ameloblastoma. Jpn Dent Sci Rev. 2021;57 (1):27-32.
  39. Zhang B, Zhang J, Huang HZ, Xu ZY, Xie HL. Expression and role of metalloproteinase-2 and endogenous tissue regulator in ameloblastoma. J Oral Pathol Med. 2010;39 (3):219-22.
  40. Kumamoto H, Ooya K. Immunohistochemical detection of MT1-MMP, RECK, and EMMPRIN in ameloblastic tumors. J Oral Pathol Med. 2006;35 (6):345-51.
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