Impact of Immunohistochemical Expression of Collagen III and MMP-14 in Odontogenic Keratocyst
DOI:
https://doi.org/10.17656/sdj.10171Keywords:
Immunohistochemical, Keratocyst, MMP14, Collagen III, SulaimaniAbstract
Objective: Immunohistochemical expression of collagen III and MMP14 was evaluated in odontogenic keratocyst, and correlated to
the clinicopathological parameters to remark on the role of these markers in the biological behavior of the cyst.
Methods: Twenty-three odontogenic keratocysts samples were collected from three pathological laboratories in Sulaimani.
Demographic information and the available radiographic investigation were recorded. The immune expression of collagen III and
MMP14 was evaluated and related to the clinicopathological variables. Chi-square test was used for analysis and a p-value of 0.05 was
cut off point for significance.
Results: Males were predominantly affected by odontogenic keratocysts lesions (69.6%), which were mainly detected in the >35 years
age groups. Keratocyst lesions were seen more frequently in the mandible (82.6%), with well-defined borders, and unilocular
appearance. Collagen III was oriented more commonly in a parallel direction (70.8%), with moderate intensity (45.8%). MMP14
revealed cytoplasmic expression in the epithelial lining of the keratocyst (100%), score 3 was the most prevalent expression (54.2%)
in the cystic wall, and prominent inflammation and epithelial separation were detected with high scoring of MMP14, which were
(57.1%) and (56.3%) respectively.
Conclusions: Both markers had independent combined roles in the cyst’s biological behavior, including the prevalent parallel direction of the collagen III, while overexpression of the MMP14 might have an impact role in the aggressive behavior of the lesion.
References
Menon S. Keratocystic odontogenic tumours:
etiology, pathogenesis and treatment revisited. J
Maxillofac Oral Surg. 2015;14(3):541-7.
Qu J, Yu F, Hong Y, Guo Y, Sun L, Li X, et al.
Underestimated PTCH1 mutation rate in sporadic
keratocystic odontogenic tumors. Oral Oncol.
;51(1):40-5.
The International Agency for Research on Cancer.
Pathology and Genetics of Head and Neck
Tumours [OP]
(Medicine):9789283224174:Medicine & Health
Science [email protected]. World Health
Organization. 2005. p. 430.
EI-Naggar AK, Chan JKC, Grandis JR, Takata
TSP. WHO Classification of Head and Neck
Tumours. WHO classification of Head and neck
Tumors 2017. 2017.p 215-220.
Krishna R, Johny J, Punathil S, and Khan NM. A
rare case of odontogenic keratocyst crossing lower
midline. Austin J Dent. 2019;6(1):11-25.
Oral and Maxillofacial Pathology - Brad W.
Neville, DDS, Douglas D. Damm, DDS, Carl M.
Allen, DDS, MSD, Angela C. Chi, DMD - Google
Books.
Bhagawati BT, Gupta M, Narang G, Bhagawati S.
Keratocystic odontogenic tumor with an ectopic
tooth in maxilla. Case Rep Dent. 2013;2013:1-4.
Kumari K, Ghosh S, Patil S, Augustine D,
Samudrala Venkatesiah S, Rao RS. Expression of
type III collagen correlates with poor prognosis in
oral squamous cell carcinoma. J Investig Clin
Dent. 2017;8(4):1-7.
D’hondt S, Guillemyn B, Syx D, Symoens S, De
Rycke R, Vanhoutte L, et al. Type III collagen
affects dermal and vascular collagen
fibrillogenesis and tissue integrity in a mutant
Col3a1 transgenic mouse model. Matrix Biol.
;70:72-83.
Nielsen MJ, Karsdal MA. Type III Collagen.
Biochemistry of Collagens, Laminins and Elastin:
Structure, Function and Biomarkers. Elsevier Inc.;
p 21–30.
Kumari K, Ghosh S, Patil S, Augustine D,
Samudrala Venkatesiah S, Rao RS. Expression of
type III collagen correlates with poor prognosis in
oral squamous cell carcinoma. J Investig Clin
Dent. 2017;8(4):1-7.
Claesson-Welsh L. How the matrix
metalloproteinase MMP14 contributes to the
progression of colorectal cancer. J Clin Invest.
;130(3):1093-5.
Linklater E, Jewett CE, Prekeris R. Polarized
membrane trafficking in development and disease:
from epithelia polarization to cancer cell invasion.
Cell Polarity Dev Dis. 2018;121-46.
Gobin E, Bagwell K, Wagner J, Mysona D,
Sandirasegarane S, Smith N, et al. A pan-cancer
perspective of matrix metalloproteases (MMP)
gene expression profile and their
diagnostic/prognostic potential. BMC Cancer.
;19(1):1-10.
Karande AM, Khandeparkar R, Vergeese CS JH.
Role of molecular markers in understanding the
tumor invasioness of ameloblastoma. Med Res
Chronicles. 2017;4(1):1-9.
Singh H, Shetty DC, Wadhwan V, Aggarwal P. A
quantitative and qualitative comparative analysis
of collagen fibers to determine the role of
connective tissue stroma on biological behavior of
odontogenic. Natl J Maxillofac Surg.
;13(1):15.
Paulo P, Santos A, Francisco C, Nonaka W, Carlos
·, Galvão Barboza A, et al. Immunohistochemical
analysis of MMP-13 and EMMPRIN in epithelial
odontogenic lesions. Eur Arch Oto-Rhino-
Laryngology. 2019;1(3):3203-11.
Waheed SA, Zaidan TF, Abdullah BH.
Odontogenic cysts and tumors of maxilla and
maxillary sinus (A clinicopathological analysis). J
Baghdad Coll Dent. 2021;33(4):38-43.
Akhtar F, Gillani SM. Clinicopathological
Analysis of Odontogenic Cysts in Iraqi Population.
J Med Chem Sci. 2022;6(4):61-5.
Hosgor H, Tokuc B, Kan B, Coskunses FM.
Evaluation of biopsies of oral and maxillofacial
lesions: A retrospective study. J Korean Assoc
Oral Maxillofac Surg. 2019;45(6):316-23.
Mohanty S, Dabas J, Verma A, Gupta S, Urs AB,
Hemavathy S. Surgical management of the
odontogenic keratocyst: A 20-year experience. Int
J Oral Maxillofac Surg. 2021;50(9):1168-76.
Al-Rawi NH, Al-Siraj AK, Majeed AH.
Comparison of osteoclastogenesis and local
invasiveness of ameloblastoma and keratocystic
odontogenic tumor. Eur J Dent. 2018;12(1):36-42.
Jung HD, Lim JH, Kim HJ, Nam W CI, Jung HD,
Lim JH, et al. Appropriate follow-up period for
odontogenic keratocyst: a retrospective study.
Maxillofac Plast Reconstr Surg. 2021;43(1):1-6.
Karaca Ç, Dere KA, Er N, Aktaş A, Tosun E,
Köseoğlu OT, et al. Recurrence rate of
odontogenic keratocyst treated by enucleation and
peripheral ostectomy retrospective case series with
up to 12 years of follow-up. Med Oral Patol Oral y
Cir Bucal. 2018;23(4):e443-8.
Malaguez GG, Munhoz EA, Rivero ERC, Rados P
V., Oliveira MG. Podoplanin expression in
odontogenic keratocysts associated or not
associated with nevoid Basal cell carcinoma
syndrome. Appl Immunohistochem Mol Morphol.
;28(7):513-7.
Shoaee S, Khazaei P, Mashhadiabbas F, Varshosaz
M, Sharifi F, Hessari H. Association between tooth
impaction and odontogenic lesions: A matched
case-control study. Med J Islam Repub Iran.
;32(1):334-7.
Kitisubkanchana J, Reduwan NH, Poomsawat S,
Pornprasertsuk-Damrongsri S,
Wongchuensoontorn C. Odontogenic keratocyst
and ameloblastoma: radiographic evaluation. Oral
Radiol 2020 371. 2020;37(1):55-65.
Sánchez-Burgos R, González-Martín-Moro J,
Pérez-Fernández E, Burgueño-García M. Clinical,
radiological and therapeutic features of
keratocystic odontogenic tumours: A study over a
decade. J Clin Exp Dent. 2014;6(3):259-64.
Jahagirdar DS. Role of Thickness of Collagen
Fibers in Odontogenic Keratocyst and Dentigerous
Cyst– A Picrosirius Red Polarized Microscopic
Study. J Med Sci Clin Res. 2017;05(09):28322-7.
Manthapuri S, Sanjeevareddygari S, Mantha H,
Oruganti R V, Reddy S, Vamshi VR. Evaluation of
biological behavior of odontogenic keratocyst and
orthokeratinized odontogenic cyst using
picrosirius red stain: A clinicopathological
retrospective study. J Dr NTR Univ Heal Sci.
;8(3):206-10.
Raj Y, Sekhar MSM, Shylaja S, Bhavani SN,
Ramanand OV, Patha S, et al. Evaluation of the
nature of collagen fibers in KCOT, dentigerous
cyst and ameloblastoma using picrosirius red
stain–A comparative study. J Clin Diagnostic Res.
;9(11):ZC0 1-4.
Aggarwal P, Saxena S. Stromal differences in
odontogenic cysts of a common histopathogenesis
but with different biological behavior: A study
with picrosirius red and polarizing microscopy.
Indian J Cancer. 2011;48(2):211-5.
Moure SP, Carrard VC, Lauxen IS, Manso PPA,
Oliveira MG, Martins MD, et al. Collagen and
Elastic Fibers in Odontogenic Entities: Analysis
Using Light and Confocal Laser Microscopic
Methods. Open Dent J. 2011;5(1):116.
Amm HM, Casimir MD, Clark DB, Sohn P MM.
Matrix metalloproteinase expression in
keratocystic odontogenic tumors and primary
cells. Connect Tissue Res. 2014;55(1):97-101.
Ribeiro Ribeiro AL, da Costa NMM, de Siqueira
AS, Brasil da Silva W, da Silva Kataoka MS,
Jaeger RG, et al. Keratocystic odontogenic tumor
overexpresses invadopodia-related proteins,
suggesting invadopodia formation. Oral Surg Oral
Med Oral Pathol Oral Radiol. 2016;122(4):500-8.
Chen KHE, Chen C, Lopez T, Radecki KC,
Bustamante K, Lorenson MY, et al. Use of a novel
camelid-inspired human antibody demonstrates
the importance of MMP-14 to cancer stem cell
function in the metastatic process. Oncotarget.
;9(50):29431-44.
Lund DK, Mouly V, Cornelison DDW. MMP-14
is necessary but not sufficient for invasion of three-
dimensional collagen by human muscle satellite Cell Physiol.
;307(2):140-9.
Fuchigami T, Ono Y, Kishida S, Nakamura N.
Molecular biological findings of ameloblastoma.
Jpn Dent Sci Rev. 2021;57(1):27-32.
Zhang B, Zhang J, Huang HZ, Xu ZY, Xie HL.
Expression and role of metalloproteinase-2 and
endogenous tissue regulator in ameloblastoma. J
Oral Pathol Med. 2010;39(3):219-22.
Kumamoto H, Ooya K. Immunohistochemical
detection of MT1-MMP, RECK, and EMMPRIN
in ameloblastic tumors. J Oral Pathol Med.
;35(6):345-51."
Downloads
Published
Issue
Section
License
Copyright (c) 2023 Helin F. Hassan, Balkis T. Garib, Dena N. Mohammad
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.
This work is licensed under 