Impact of Immunohistochemical Expression of Collagen III and MMP-14 in Odontogenic Keratocyst


  • Helin F. Hassan Oral Diagnosis Department, College of Dentistry, University of Sulaimani, Kurdistan Region, Iraq. Author
  • Balkis T. Garib Oral Diagnosis Department, College of Dentistry, University of Sulaimani, Kurdistan Region, Iraq. Author
  • Dena N. Mohammad Oral Diagnosis Department, College of Dentistry, University of Sulaimani, Kurdistan Region, Iraq. Author



Immunohistochemical, Keratocyst, MMP14, Collagen III, Sulaimani


Objective: Immunohistochemical expression of collagen III and MMP14 was evaluated in odontogenic keratocyst, and correlated to
the clinicopathological parameters to remark on the role of these markers in the biological behavior of the cyst.

Twenty-three odontogenic keratocysts samples were collected from three pathological laboratories in Sulaimani.
Demographic information and the available radiographic investigation were recorded. The immune expression of collagen III and
MMP14 was evaluated and related to the clinicopathological variables. Chi-square test was used for analysis and a p-value of 0.05 was
cut off point for significance.

Males were predominantly affected by odontogenic keratocysts lesions (69.6%), which were mainly detected in the >35 years
age groups. Keratocyst lesions were seen more frequently in the mandible (82.6%), with well-defined borders, and unilocular
appearance. Collagen III was oriented more commonly in a parallel direction (70.8%), with moderate intensity (45.8%). MMP14
revealed cytoplasmic expression in the epithelial lining of the keratocyst (100%), score 3 was the most prevalent expression (54.2%)
in the cystic wall, and prominent inflammation and epithelial separation were detected with high scoring of MMP14, which were
(57.1%) and (56.3%) respectively.

Both markers had independent combined roles in the cyst’s biological behavior, including the prevalent parallel direction of the collagen III, while overexpression of the MMP14 might have an impact role in the aggressive behavior of the lesion.


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How to Cite

Impact of Immunohistochemical Expression of Collagen III and MMP-14 in Odontogenic Keratocyst. (2023). Sulaimani Dental Journal, 10(2), 9.